APPLICATIONS OF XYLANASE, LACCASE ENZYME AND HIGH POWER ULTRASOUND ON DIFFERENT NON-WOOD PLANTS

Abstract

The purpose of this bachelor`s thesis was to analyze the effect of properly applied microbial enzymes on non-wood plants delignification, improved fibre flexibility, fibrillation, removal of xylan and facilitated contaminant. The enzyme plays important role in digestion of fibres and removes lignin content of pulp. In the experimental part, two different non-woody plants were experimented: flax, straw. The enzymes Laccase and Xylanase were used. The amount of enzyme was tested in four part: 0.006g,0.042g,0.048g,0,003g.The experiment was carried out with this different amount of enzyme using flax and straw(two non-wood plant).The different parameter were used to analyze their results : change in temperature, concentration of enzyme, change of ultrasound time and change in Ph level. The aim of work was to remove lignin content from non-wood plants with use of enzyme and ultrasound treatment .The parameter like changes in temperature, concentration of enzymes, ultrasound time and pH values were used in this experiment. These play an important role in separation and digestion of much better fibres.