Optimization of 2,3-butanediol production in a bioreactor by Bacillus amyloliquefaciens
Manninen, Elina (2015)
Tampereen ammattikorkeakoulu
2015
Creative Commons Attribution-NonCommercial-ShareAlike 1.0 Suomi
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:amk-2015113018456
https://urn.fi/URN:NBN:fi:amk-2015113018456
Tiivistelmä
Due to the depleting fossil fuel reserves and the ever changing oil prices, the production of 2,3-butanediol has shifted towards more biological methods. Current studies are experimenting with various strains of bacteria and carbon sources to find the optimal production method. Finding the right balance in the production could make it possible to produce 2,3-butanediol in a larger scale. For this purpose, the production of 2,3-butanediol by the bacteria Bacillus amyloliquefaciens was optimized.
Three experiments were performed in a 15 litre fermenter during a three-week period at the Ostfalia University of Applied Sciences in Wolfenbüttel, Germany. Each of the tests had the same starting medium and measured parameters with the addition of one or two feedings of sucrose. By adjusting the different parameters, such as the oxygen percentage in the medium, air flow and pH, an aerobic growth phase and an aerobic production phase could be achieved.
The results of the optimization of the production of 2,3-butanediol show that sucrose can be used to replace glucose as the main carbon source. However, there was no significant increase in the production of 2,3-butanediol with the addition of the feeding. While adjusting the availability of oxygen a clear growth and production phase can be distinguished. The results were positive although there is need for further experiments with more advanced analytical methods, and upgraded measuring devices.
Three experiments were performed in a 15 litre fermenter during a three-week period at the Ostfalia University of Applied Sciences in Wolfenbüttel, Germany. Each of the tests had the same starting medium and measured parameters with the addition of one or two feedings of sucrose. By adjusting the different parameters, such as the oxygen percentage in the medium, air flow and pH, an aerobic growth phase and an aerobic production phase could be achieved.
The results of the optimization of the production of 2,3-butanediol show that sucrose can be used to replace glucose as the main carbon source. However, there was no significant increase in the production of 2,3-butanediol with the addition of the feeding. While adjusting the availability of oxygen a clear growth and production phase can be distinguished. The results were positive although there is need for further experiments with more advanced analytical methods, and upgraded measuring devices.