Upscaling production of 2,3-Butanediol by Bacillus amyloliquefaciens utilising waste washing treatment effluent from municipal solid waste as substrate
Prayoga, Adhitya; Walpole, Robert (2017)
Tampereen ammattikorkeakoulu
2017
All rights reserved
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:amk-2017082314508
https://urn.fi/URN:NBN:fi:amk-2017082314508
Tiivistelmä
The hypothesis that 2,3-Butanediol can be produced by Bacillus amyloliquefaciens, using water used to wash municipal solid waste was first investigated by Liebig & Gerlitz. Their work gave promising results and was a prime candidate to be replicated and verified, which was done by a project team. The team went further and included up-scaling as a part of their scope. This current paper implements their recommendations and further strives to increase the yield by methodological improvement, while continuously increasing the process scale.
Results were successful with a yield of 295,66 mg l-1 2,3-butanediol achieved using a facility with a maximum TRL level of 4. The timing of extraction is crucial due to the reverse transformation of 2,3-butanediol to acetoin. Several sources of inhibition were identified, especially pH, and these must be considered in future production. The current maximum yield was not achieved under ideal parameters, thus leaving room for the further exploration of upscaling under optimum conditions to improve yield and volumetric production.
Results were successful with a yield of 295,66 mg l-1 2,3-butanediol achieved using a facility with a maximum TRL level of 4. The timing of extraction is crucial due to the reverse transformation of 2,3-butanediol to acetoin. Several sources of inhibition were identified, especially pH, and these must be considered in future production. The current maximum yield was not achieved under ideal parameters, thus leaving room for the further exploration of upscaling under optimum conditions to improve yield and volumetric production.