The transformed products of Disulfoton contribute to Cholinesterase inhibition activity on human

Abstract

The purpose of this study is to evaluate the toxicity (cholinesterase inhibition activity) of a solution containing a pesticide (disulfoton) during the chlorination process and identify the transformation products contributing to the observed toxicity. This study was performed by preparing a batch of chlorinated samples, conducting assay test with cholinesterase from human blood and construct quantification analysis by LC/MS. As a result, the toxicity of the solution containing disulfoton increased by chlorination. Disulfoton transformed mostly into demeton-s-sulfone, with demeton-s-sulfoxide was the intermediate oxidation product. Other transformation product(s) than the oxon form contributed to the increased toxicity. In conclusion, possible future work should be implemented in order to observe more precise and deeper results.

The thesis topic belongs to Environmental Risk Engineering laboratory, Hokkaido University. The permission for doing this thesis work was given by Professor. Matsushita – the associate professor of the laboratory. This research was supported and assisted by the Environmental Risk Engineering laboratory, Hokkaido University.