Development of an in vitro method to determine the binding capacity of bentonite and diatomite mycotoxin binders with deoxynivalenol
Ilomäki, Henry (2021)
2021
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Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:amk-2021121325660
https://urn.fi/URN:NBN:fi:amk-2021121325660
Tiivistelmä
The aim of this thesis was to examine mycotoxin binders´ adsorption capacity of deoxynivalenol and aflatoxin B1 mycotoxins in a single-concentration experiment. This study is part of the Bioproton Europe Oy project, which aims to explore the efficacy of some mineral binders.
The experiment was conducted with two different mycotoxin binders suitable for mycotoxins adsorption in gastrointestinal simulated conditions. Unbound deoxynivalenol was analysed with high-performance liquid chromatography using a variable wavelength detector (VWD). The choice of these methods for analyzing unbound deoxynivalenol contents was based on previous literature.
In this experiment unbound deoxynivalenol content was analyzed. The aflatoxin samples were not analyzed due to insufficient equipment; they were sent to an external laboratory for further analysis and thus, are missing in this thesis. Only the results of deoxynivalenol are discussed and they show that bentonite-based and the two diatomite-based mycotoxin binders did not adsorb the deoxynivalenol. In this study the limit of detection (LOD) and the limit of quantification (LOQ) were 2.22 µg/mL and 7.39 µg/mL, respectively.
The experiment was conducted with two different mycotoxin binders suitable for mycotoxins adsorption in gastrointestinal simulated conditions. Unbound deoxynivalenol was analysed with high-performance liquid chromatography using a variable wavelength detector (VWD). The choice of these methods for analyzing unbound deoxynivalenol contents was based on previous literature.
In this experiment unbound deoxynivalenol content was analyzed. The aflatoxin samples were not analyzed due to insufficient equipment; they were sent to an external laboratory for further analysis and thus, are missing in this thesis. Only the results of deoxynivalenol are discussed and they show that bentonite-based and the two diatomite-based mycotoxin binders did not adsorb the deoxynivalenol. In this study the limit of detection (LOD) and the limit of quantification (LOQ) were 2.22 µg/mL and 7.39 µg/mL, respectively.