Establishing pollen collection for TAMK
Zhao, Chuanhui (2012)
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:amk-2012121319400
https://urn.fi/URN:NBN:fi:amk-2012121319400
Tiivistelmä
This thesis was focused on preparation of microscopic samples for further scientific and educational use. The work for the thesis included collecting pollen samples from different species during blossom seasons, preserving the pollen samples, making microscopic samples, and observing the pollen samples through microscope. Purpose of the thesis is to serve as handout for laboratory work on pollen samples and other work continuing with the pollen collection. Additionally, there was also seasonal work in helping identify airborne pollen samples for allergic monitoring in Finland. The work was proceeded from beginning of May till beginning of September in Tampere, when there were allergenic pollen grains suspending in the atmosphere, and meanwhile pollen collection from the plants was carried out as well.
To better perform the pollen sample preparation project and the seasonal assisting work, some scientific theories and information were studied and researched as well, such as allergic rhinitis which is caused by the allergens such as pollen, animal dander, etc.; blossom times (starting from April and ending in September generally) and appearances of the species (by previewing pictures of the species of various angles); general information about plant (Classified as bryophytes, ferns, and seed plants) and pollination through which seed plants are self-pollinated or cross-pollinated; importance of pollen to human being (such as being used in historic research and pollinator research, etc.); etc. Both dry and moist (frozen) samples were tested for the preparation, in which moist ones were found more efficient and convenient for this purpose. The glue used for stabilizing the pollen grains onto the microscopic slides was prepared in the laboratory successfully as well, after several different approaches.
Preparation of the microscopic samples was the main and most challenging experi-mental part of the whole project, which was quite interesting at the same time. The recipe used for preparing the gel was useful for the microscopic samples; more recipes could be approached and tested though. The microscopic samples prepared in the laboratory were from species included Alnus, Artemisia vulgaris, Betula, Chenopodium album, Corylus, Epilobium, and Rosa majalis, for which the microscopic photos were taken as well. The checklist and instruction of entire procedure of preparing the micro-scopic samples from plant pollen sampling to microscopic photography are included in the thesis as well.
To better perform the pollen sample preparation project and the seasonal assisting work, some scientific theories and information were studied and researched as well, such as allergic rhinitis which is caused by the allergens such as pollen, animal dander, etc.; blossom times (starting from April and ending in September generally) and appearances of the species (by previewing pictures of the species of various angles); general information about plant (Classified as bryophytes, ferns, and seed plants) and pollination through which seed plants are self-pollinated or cross-pollinated; importance of pollen to human being (such as being used in historic research and pollinator research, etc.); etc. Both dry and moist (frozen) samples were tested for the preparation, in which moist ones were found more efficient and convenient for this purpose. The glue used for stabilizing the pollen grains onto the microscopic slides was prepared in the laboratory successfully as well, after several different approaches.
Preparation of the microscopic samples was the main and most challenging experi-mental part of the whole project, which was quite interesting at the same time. The recipe used for preparing the gel was useful for the microscopic samples; more recipes could be approached and tested though. The microscopic samples prepared in the laboratory were from species included Alnus, Artemisia vulgaris, Betula, Chenopodium album, Corylus, Epilobium, and Rosa majalis, for which the microscopic photos were taken as well. The checklist and instruction of entire procedure of preparing the micro-scopic samples from plant pollen sampling to microscopic photography are included in the thesis as well.