Extraction possibilities of lipid fraction and authentication assessment of chaga (Inonotus obliquus)
Upska, Karina; Klavins, Linards; Radenkovs, Vitalijs; Nikolajeva, Vizma; Faven, Leena; Isosaari, Eveliina; Lauberts, Maris; Busa, Lauma; Viksna, Arturs; Klavins, Maris (2022)
Springer Science and Business Media LLC
2022
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Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi-fe2022021719617
https://urn.fi/URN:NBN:fi-fe2022021719617
Tiivistelmä
Chaga (Inonotus obliquus) (Fr.) Pilát is a black perennial fungus that grows on adult birch trunk and
traditionally been used as a health prevention remedy in different countries. The lipophilic compounds
of chaga were isolated applying conventional (Soxhlet), non-conventional (ultra-sound assisted,
accelerated solvent) and environmentally friendly (supercritical fluid) extraction methods utilizing both
polar and non-polar solvents. For authentication of chaga samples, isotope ratio mass spectrometry
(IRMS) and multi-elemental analysis was performed in this study. The yield and profile of lipids, sterols,
and triterpenoids of various origins were discussed and potential application in terms of antimicrobial
activity against pathogenic and opportunistic pathogenic microorganisms demonstrated. The results
showed that CO2 extraction delivered comparable to conventional extraction techniques amount of target
compounds with a safer profile and therefore could represent the future for sustainable industrial-scale
production of biologically active ingredients with antimicrobial activity. Authentication of chaga
allowed to establish the quality parameters and properties of resources that will be useful for industries.
traditionally been used as a health prevention remedy in different countries. The lipophilic compounds
of chaga were isolated applying conventional (Soxhlet), non-conventional (ultra-sound assisted,
accelerated solvent) and environmentally friendly (supercritical fluid) extraction methods utilizing both
polar and non-polar solvents. For authentication of chaga samples, isotope ratio mass spectrometry
(IRMS) and multi-elemental analysis was performed in this study. The yield and profile of lipids, sterols,
and triterpenoids of various origins were discussed and potential application in terms of antimicrobial
activity against pathogenic and opportunistic pathogenic microorganisms demonstrated. The results
showed that CO2 extraction delivered comparable to conventional extraction techniques amount of target
compounds with a safer profile and therefore could represent the future for sustainable industrial-scale
production of biologically active ingredients with antimicrobial activity. Authentication of chaga
allowed to establish the quality parameters and properties of resources that will be useful for industries.