Preparation of PS-PGMA-DVB microspheres
Peng, Siyuan (2022)
2022
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Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:amk-2022060716323
https://urn.fi/URN:NBN:fi:amk-2022060716323
Tiivistelmä
Chromatography, also known as chromatographic analysis, is a separation and analysis method. It is widely used in the fields of analytical chemistry, organic chemistry, and biochemistry. To achieve the successful separation of components in chromatography, it is critical to select the appropriate packing material, as this directly affects the separation efficiency and accuracy of the chromatography.
With the rapid development of proteomics and metabolomics, many polar and hydrophilic substances such as polar metabolites, carbohydrates or peptides are difficult to separate effectively by conventional chromatography, and this is why hydrophilic interaction liquid chromatography (HILIC) is becoming increasingly significant. However, the variety of hydrophilic packings suitable for this chromatography is still relatively small. Therefore, it is critical to develop new chromatographic packings with high hydrophilicity, high selectivity, and broader applicability.
In this paper, a novel polystyrene-based hydrophilic chromatographic packing PS-PGMA-DVB was synthesised by modifying the functional group of PS microspheres. PS microspheres were used as the substrate in the synthesis process, DVB was used as the crosslinking agent, and PGMA was successfully linked to the PS microspheres by the seed swelling polymerisation method. However, the characteristics and performance of the PS-PGMA-DVB were not evaluated in this experiment. Therefore, further study on its properties is needed.
With the rapid development of proteomics and metabolomics, many polar and hydrophilic substances such as polar metabolites, carbohydrates or peptides are difficult to separate effectively by conventional chromatography, and this is why hydrophilic interaction liquid chromatography (HILIC) is becoming increasingly significant. However, the variety of hydrophilic packings suitable for this chromatography is still relatively small. Therefore, it is critical to develop new chromatographic packings with high hydrophilicity, high selectivity, and broader applicability.
In this paper, a novel polystyrene-based hydrophilic chromatographic packing PS-PGMA-DVB was synthesised by modifying the functional group of PS microspheres. PS microspheres were used as the substrate in the synthesis process, DVB was used as the crosslinking agent, and PGMA was successfully linked to the PS microspheres by the seed swelling polymerisation method. However, the characteristics and performance of the PS-PGMA-DVB were not evaluated in this experiment. Therefore, further study on its properties is needed.