Optimization of methods for chromatography equipment in organic chemistry manufacturing process
Kontto, Saara (2025)
2025
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Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:amk-2025121636984
https://urn.fi/URN:NBN:fi:amk-2025121636984
Tiivistelmä
The purpose of this work was to optimize and transfer the chromatographic purification methods of a widely used semi-product in Radiometer Turku Oy's production to the CombiFlash EZPrep system. The goal was to improve production efficiency and occupational safety.
The research method used was functional research, in which a continuous cyclic process was applied to develop effective parameters for the purification methods of two different compounds. Thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analyses were used to verify and confirm the purification.
At the beginning of the optimization process, the chromatographic purification method resulted in a compound that separated too quickly and in two parts. Based on the results, various methanol concentrations were tested during the purification run and in the pre-wetting of the sample cartridge. As a result, optimized parameters were developed and transferred for the chromatographic purification of two different compounds using the CombiFlash EZPrep system. The goal of improving production efficiency and occupational safety was achieved, as the purification run performed with the device saved working time and reduced the amount of solvents used in the process.
The research method used was functional research, in which a continuous cyclic process was applied to develop effective parameters for the purification methods of two different compounds. Thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analyses were used to verify and confirm the purification.
At the beginning of the optimization process, the chromatographic purification method resulted in a compound that separated too quickly and in two parts. Based on the results, various methanol concentrations were tested during the purification run and in the pre-wetting of the sample cartridge. As a result, optimized parameters were developed and transferred for the chromatographic purification of two different compounds using the CombiFlash EZPrep system. The goal of improving production efficiency and occupational safety was achieved, as the purification run performed with the device saved working time and reduced the amount of solvents used in the process.