Exploring the potential of bacterial ureases : utilizing Corynebacterium urealyticum
Conroy, Isis (2024)
2024
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Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:amk-2024060320286
https://urn.fi/URN:NBN:fi:amk-2024060320286
Tiivistelmä
This thesis investigated the ureolytic activity of Corynebacterium urealyticum and compared it to Canavalia ensiformis (jack bean), a traditional plant-based urease source. The potential benefits of utilizing bacteria-based urease include enhanced applicability in sanitation system research, biomedicine, and environmental management due to its alignment with real-world conditions where bacterial ureases are prevalent. The objectives of this study were to evaluate the urease activity of Corynebacterium urealyticum, compare its ureolytic activity with that of Canavalia ensiformis, and optimize the cultivation conditions for C. urealyticum to achieve consistent and reliable measurements.
Experimental findings revealed that C. urealyticum urease activity averaged 287,71 U/mL under the given experimental conditions, demonstrating comparable efficiency to plant-derived ureases. Although crystallized jack bean urease could reach much higher concentrations, making direct comparisons challenging, bacterial ureases could be equally effective if similarly crystallized.
The findings advocate for the expanded use of bacterial ureases in urea degredation processes, enhancing the accuracy of testing conditions by aligning them more closely with real-world conditions. The extended activity of live bacterial urease, as demonstrated in this study, suggests its potential for long-term applications where continuous urea breakdown is needed.
Experimental findings revealed that C. urealyticum urease activity averaged 287,71 U/mL under the given experimental conditions, demonstrating comparable efficiency to plant-derived ureases. Although crystallized jack bean urease could reach much higher concentrations, making direct comparisons challenging, bacterial ureases could be equally effective if similarly crystallized.
The findings advocate for the expanded use of bacterial ureases in urea degredation processes, enhancing the accuracy of testing conditions by aligning them more closely with real-world conditions. The extended activity of live bacterial urease, as demonstrated in this study, suggests its potential for long-term applications where continuous urea breakdown is needed.