Validation of Ion Chromatographic method for simultaneous quantification of Mg2+, K+, NH4+ and Na+ ions in food salt
Nobre, Ingrid (2012)
Metropolia Ammattikorkeakoulu
2012
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Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:amk-201503123098
https://urn.fi/URN:NBN:fi:amk-201503123098
Tiivistelmä
Salt is a vital substance for all of us. Usually salt contains sodium, which can be
very dangerous if consumed in high amounts. Because of this issue, some salt
companies have been developing alternative salts with less sodium.
The aim of this project was to ascertain if the Metrohm Ion Chromatographic method and the device itself are adequate for the determination of ammonium, potassium and magnesium ions in salt samples provided by Smart Salt Inc. Another aim was to determine basic data for the foundation of method validation. Ion chromatography is a simple and fast method for separation and determination of ions based on their charge, and it is suitable for fast analyze and especially for environmental analysis.
Smart salt Inc. has invented a co-crystallized triple salt of magnesium-, potassium and ammonium chloride. The triple salt is blended with sodium and potassium chloride in vari- ous ratios and used in food products to effect a sodium reduction of up to 60%. The reason for analyzing these positive ions is to verify that all the molar ratios remain the same after the manufacturing process of the product.
Five standard solutions of four cations (Mg2+, K+, NH4+ and Na+ ions) are used to specify each peak of the wanted ions. The linearity test after calibraion had a satisfactory correlation coeficiente of R2>0.999.
The parameters which have been determined for method validation are linearity,
accuracy, precision, injection repeatability, and t-test for error significance.
It was concluded on the basis of the results that the method used for validation
exhibits evidence for systematic error at the 95 % confidence level. This systematic error is probably due to the calibration period of 6 months between Smart Salt analysis and the analyst samples analysis. Therefore, this method cannot be used. The second calibration yielded more accurate results.
very dangerous if consumed in high amounts. Because of this issue, some salt
companies have been developing alternative salts with less sodium.
The aim of this project was to ascertain if the Metrohm Ion Chromatographic method and the device itself are adequate for the determination of ammonium, potassium and magnesium ions in salt samples provided by Smart Salt Inc. Another aim was to determine basic data for the foundation of method validation. Ion chromatography is a simple and fast method for separation and determination of ions based on their charge, and it is suitable for fast analyze and especially for environmental analysis.
Smart salt Inc. has invented a co-crystallized triple salt of magnesium-, potassium and ammonium chloride. The triple salt is blended with sodium and potassium chloride in vari- ous ratios and used in food products to effect a sodium reduction of up to 60%. The reason for analyzing these positive ions is to verify that all the molar ratios remain the same after the manufacturing process of the product.
Five standard solutions of four cations (Mg2+, K+, NH4+ and Na+ ions) are used to specify each peak of the wanted ions. The linearity test after calibraion had a satisfactory correlation coeficiente of R2>0.999.
The parameters which have been determined for method validation are linearity,
accuracy, precision, injection repeatability, and t-test for error significance.
It was concluded on the basis of the results that the method used for validation
exhibits evidence for systematic error at the 95 % confidence level. This systematic error is probably due to the calibration period of 6 months between Smart Salt analysis and the analyst samples analysis. Therefore, this method cannot be used. The second calibration yielded more accurate results.